bioRxivCell BiologyPreprint
Tissue-specific endothelial cell heterogeneity in human type 2 diabetes
Alexander-Francisco BrunsiD, Oliver Ian BrowniD, Hema ViswambharaniD, Klaus Karl WitteiD, Sam StrawiD, John GierulaiD, Chew Weng ChengiD, Katherine BridgeiD, Natalie J HaywoodiD, Anna SkromnaiD, Natallia MakavaiD, Lauren F Daly, Matthew Bourn, Jeanne F Rivera, Nora Almuhim, Michael DrozdiD, David J. BeechiD, Lee D. RobertsiD, Khalid M. NaseemiD, Richard M. CubboniD, Mark T. KearneyiD
Abstract
Transcriptomic heterogeneity of microvascular endothelial cells (MVEC) within subcutaneous adipose tissue (SAT) in preclinical models of obesity/type 2 diabetes mellitus (T2DM) is well-established. Here, we examined if such heterogeneity exists between two canonical insulin-target tissues, SAT and skeletal muscle (SkM). Characterisation of SAT and SkM MVEC from humans with T2DM demonstrated that SAT, but not SkM, exhibited cellular stress at transcriptomic, morphological, and protein levels.
- organism: human
- assay: bulk RNA-seq
- research focus: cell type annotation
- research focus: differential expression
- approach: statistical model
- Citations
- 0
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