Learned response dynamics reflect stimulus timing and encode temporal expectation violations in superficial layers of mouse V1

Abstract

The ability to recognize ordered event sequences is a fundamental component of sensory cognition and underlies the capacity to generate temporally specific expectations of future events based on previous experience. Various lines of evidence suggest that the primary visual cortex participates in some form of predictive processing, though many details remain ambiguous. Here we use two-photon calcium imaging in layer 2/3 (L2/3) of the mouse primary visual cortex (V1) to study changes in neural activity under a multi-day sequence learning paradigm with respect to prediction error responses, stimulus encoding, and time. We find increased neural activity at the time an expected, but omitted, stimulus would have occurred but no significant prediction error responses following an unexpected stimulus substitution. Sequence representations became sparser and less correlated with training, although these changes had no effect on decoding accuracy of stimulus identity or timing. Additionally, we find that experience modifies the temporal structure of stimulus responses to produce a bias towards predictive stimulus-locked activity. Finally, we observe significant temporal structure during intersequence rest periods that was largely unchanged by training.