A. Barkan

Translational activation by a synthetic PPR protein elucidates control of psbA translation in Arabidopsis chloroplasts

A synthetic PPR protein substitutes for HCF173, elucidating its role in activating psbA translation and demonstrating synthetic PPRs' ability to control chloroplast gene expression.

Posttranscriptional tuning of gene expression over a large dynamic range in synthetic tobacco chloroplast operons

The paper investigates how different UTRs and tRNA elements affect gene expression in synthetic tobacco chloroplast operons, showing a wide range of expression levels controlled by PPR10.

Correlated retrograde and developmental regulons implicate multiple retrograde signals as coordinators of chloroplast development in maize

Transcriptomes of non-photosynthetic maize mutants reveal three plastid signals coordinating photosynthetic differentiation during leaf development.

Ribosome profiling elucidates differential gene expression in bundle sheath and mesophyll cells in maize

This study uses ribosome profiling and RNA-seq to analyze translational efficiency and gene expression differences between bundle sheath and mesophyll cells in maize, identifying 182 genes with cell-type dependent translation.

In vivo stabilization of endogenous chloroplast RNAs by customized artificial pentatricopeptide repeat proteins

This study demonstrates that artificial PPR proteins can stabilize specific chloroplast RNAs in vivo by mimicking natural PPR proteins' functions.

Light-induced psbA translation in plants is triggered by photosystem II damage via an assembly-linked autoregulatory circuit

The paper identifies light-induced D1 damage as the signal triggering psbA ribosome recruitment and global translation elongation in plants, revealing an autoregulatory mechanism linking D1 synthesis to PSII repair.