Rapid and site-specific deep phosphoproteome profiling by data-independent acquisition (DIA) without the need for spectral libraries
D. B. Bekker-Jensen, O. Bernhardt, A. Hogrebe, A. Martinez del Val, L. Verbeke, T. Gandhi, C. D. Kelstrup, L. Reiter, J. V. Olsen
This paper introduces a DIA-based phosphoproteomics method that achieves high sensitivity and reproducibility without spectral libraries, demonstrating its scalability in analyzing kinase inhibitor effects on EGF signaling.
bioRxiv · Jun 3, 2019Biochemistry