BiochemistrybioRxiv
Rapid and site-specific deep phosphoproteome profiling by data-independent acquisition (DIA) without the need for spectral libraries
D. B. Bekker-Jensen, O. Bernhardt, A. Hogrebe…·J. V. Olsen
This paper introduces a DIA-based phosphoproteomics method that achieves high sensitivity and reproducibility without spectral libraries, demonstrating its scalability in analyzing kinase inhibitor effects on EGF signaling.
DOI 10.1038/s41467-020-14609-1