A multiplexed work-flow for absolute quantification of Klebsiella oxytoca Nif proteins
C. J. Waite, A. Lindstrom-Battle, M. Bennett, M. Buck, J. Schumacher
This study develops a high-throughput pipeline to quantify all twenty Nif proteins in Klebsiella oxytoca using MRM-MS and QconCAT standards, revealing temporal regulation and abundance disparities.
bioRxiv · Apr 27, 2020 · PreprintBiochemistry