Methods for the targeted sequencing and analysis of integrons and their gene cassettes from complex microbial communities
T. M. Ghaly, A. Penesyan, A. Pritchard, Q. Qi, V. Rajabal, S. G. Tetu, M. R. Gillings
This paper presents standardized methods for PCR amplification, sequencing, and validation of integron gene cassettes from complex microbial communities, demonstrating their ability to recover diverse functional elements including antibiotic resistance genes.
bioRxiv · Sep 8, 2021Microbiology