Late-Stage Large Extracellular Vesicles Reprogram CHO Cell Metabolism in a Glutamine-Dependent Mode and Promote Antibody-Productivity to Cell-Growth Tradeoff
Hong B Nguyen, Nikola G. Malinov, Alekhya Puttagunta, Kelvin H. Lee, Eleftherios Terry Papoutsakis
This study shows that late-stage large extracellular vesicles (LgEVs) from CHO cells reduce growth but increase antibody productivity, with glutamine modulating these effects through metabolic and miRNA mechanisms.
bioRxiv · Jun 29, 2026 · PreprintCell Biology