Target binding triggers hierarchical phosphorylation of human Argonaute-2 to promote target release
B. Bibel, E. Elkayam, S. Silletti, E. A. Komives, L. Joshua-Tor
The paper shows that miRNA-loaded Ago2 binding to target RNA primes its EI for CK1-mediated phosphorylation, which facilitates target release through electrostatic effects.
bioRxiv · Jan 6, 2022Biochemistry