Protein deuteration via algal amino acids to overcome proton back-exchange for fast-MAS solid-state NMR of large proteins
H. Aucharova, A. Klein, S. Medina Gomez, B. Soeldner, S. K. Vasa, R. Linser
The paper demonstrates that deuteration via deuterated amino acids improves spectral resolution for large proteins in solid-state NMR by avoiding proton back-exchange issues.
bioRxiv · Jan 7, 2024 · PreprintBiochemistry