HEx: a heterologous expression platform for the discovery of fungal natural products
Colin J. B. Harvey, Man‐Cheng Tang, Ulrich Schlecht, Joe Horecka, Curt R. Fischer, Hsiao‐Ching Lin, Jian Li, Brian Naughton, J. Michael Cherry, Molly Miranda, Yong Fuga Li, Angela M. Chu, James R Hennessy, Gergana A Vandova, Diane O. Inglis, , Maureen E. Hillenmeyer
A yeast-based refactoring platform (HEx) with new inducible promoters, an engineered host strain, and homology-based assembly expressed 41 cryptic fungal gene clusters; 22 yielded non-native compounds.
Science Advances · Apr 11, 2018Peer-reviewedSynthetic Biology✦ In-depth digest