flow cytometry

Circulating CD4 regulatory T cells are broadly preserved in Parkinson's disease despite altered HELIOS expression

This study examines circulating CD4 regulatory T cells in Parkinson's disease and finds they are largely preserved in phenotype and function, though show reduced HELIOS expression in naive subsets.

Deep phenomics of African green monkey and Baboon PBMCs using a 30-marker spectral flow cytometry panel

The paper develops a 30-marker spectral flow cytometry panel to profile immune cell subsets in African green monkey and baboon PBMCs, focusing on T cell subsets, cytokines, and activation markers.

A novel KIR3DL2-directed CAR T-cell therapy for cutaneous and systemic T-cell lymphomas

This paper develops a KIR3DL2-directed CAR T-cell therapy for T-cell lymphomas, showing preclinical efficacy and safety in xenograft models.

Robust T and B cell activation is associated with rapid clearance of infectious SARS-CoV-2

The paper identifies immune cell activation patterns associated with rapid clearance of infectious SARS-CoV-2 in both unvaccinated and vaccinated individuals.

Targeting NF-κB prevents Schwann cell reprogramming and plexiform neurofibroma initiation in mouse model of Nf1

Marker-specific pixel attribution for single-cell protein quantification with PixAQ

PixAQ improves single-cell protein quantification in imaging mass cytometry by assigning pixel signals to specific markers using spatial and intensity models.

Deep phenomics of human PBMCs using a 31-marker spectral flow cytometry panel

The paper develops a 31-marker spectral flow cytometry panel to simultaneously profile functional cytokines, AIMs, and FoxP3+ Tregs in human PBMCs under different conditions.

A Reproducible Pre-Transplant γδ T-cell Effector Signature Reveals Immune-Metabolic Programs Associated with Kidney Allograft Rejection

The paper identifies a pre-transplant γδ T-cell effector signature associated with kidney allograft rejection using multi-omics data from transplant recipients.

Serial intravascular labeling resolves dynamic decidual NK residency and gestational remodeling toward T-bet-Low states

This study uses serial intravascular labeling to show dynamic changes in decidual NK cell residency and T-bet expression during pregnancy.

Quantum Barcoding Bridges High-Dimensional Immune Phenotyping with Signaling Responses across Species

The paper benchmarks Quantum Barcoding against mass cytometry in human and rhesus macaque blood, integrating data to reveal conserved and divergent signaling responses across species.

Preserved Type 2 Immune Cell Plasticity in Human Obesity and Differential Immune Reconstitution After Bariatric Surgery

This study examines immune cell changes in obesity and after bariatric surgery, finding preserved type 2 immune cell plasticity and differential immune recovery.

HIV-1 infection does not confer intrinsic resistance to cell death induced by cytotoxic T lymphocytes

The paper assesses whether HIV-1 infection confers resistance to CTL-mediated cell death and finds no intrinsic resistance, with survival differences attributed to MHC class I downregulation.

Synthetic auxotrophy reveals metabolic regulation of plasma cell generation, affinity maturation, and cytokine receptor signaling

The paper shows that glutaminolysis and mitochondrial metabolism are conditionally crucial for B cell differentiation and cytokine signaling, revealing metabolic regulation of plasma cell generation and cytokine receptor signaling.

Adapting flow cytometry for studying immune cell seasonality in wild migratory bats

The paper adapts flow cytometry for field studies of immune cell seasonality in wild bats, revealing seasonal changes in immune cell populations.

CDH1 loss remodels gene expression and lineage identity in human mammary epithelial cells

This study shows that CDH1 loss in human mammary epithelial cells remodels gene expression and lineage identity toward a luminal progenitor-like state, contributing to ILC oncogenesis.

Modeling competitive transplantation using HLA-mismatched human hematopoietic stem cells

This paper introduces a competitive transplantation method using HLA-mismatched human hematopoietic stem cells in humanized mice to study repopulating capacity and track graft dynamics.

Beyond the Billion: Dose-Response Immunophenotyping and Machine Learning Classification of Live versus Heat-Inactivated Gram-Positive Probiotic Strains in Human Peripheral Blood Mononuclear Cells

This study challenges assumptions about probiotic efficacy by showing heat-treated strains can match live strains in immunological activity at higher doses, using flow cytometry and machine learning to classify probiotic strains.

Engineered NKG2C+ NK-like T cells exhibit superior antitumor efficacy while mitigating cytokine release syndrome

This study shows that engineered NKG2C+ NK-like T cells have better antitumor effects and lower cytokine release syndrome risk than conventional CAR-T and CAR-NK cells.

Profiling peripheral immune cells in Parkinsons disease: A Scoping Review

This review synthesizes findings on peripheral immune cell changes in Parkinson's disease, identifying common immune signatures and methodological inconsistencies across studies.

Polyhydroxyalkanoate carbonosomes impact the activity and viability of growth-arrested Pseudomonas aeruginosa during nutrient fluctuations and tobramycin exposure

This study investigates how carbonosomes in Pseudomonas aeruginosa affect cell viability and antibiotic sensitivity during nutrient fluctuations and tobramycin exposure.

Multimodal Profiling of Repair-Associated Immune Dynamics in a Mouse Model of Menstruation

This study examines immune dynamics during menstruation in mice, identifying monocytes, macrophages, and neutrophils as key players in endometrial repair and highlighting thrombospondin 1 and secreted phosphoprotein 1 as potential therapeutic targets.

Ion Channel Nano-Diagnostics for ER+ Breast Cancer

This paper develops GAT1508-coated gold nanoparticles for detecting ER+ breast cancer cells through specific binding to GIRK1 channels, validated by flow cytometry and electrophysiology.

Innate immune sensing of cell traversal by Plasmodium sporozoites drives protective T cell responses

The paper shows that Plasmodium sporozoites trigger innate immune responses via cell traversal, which is essential for activating protective CD8+ T cells.

Elevated Plasmodium falciparum sexual conversion in HbAC and HbAS red blood cells in naturally infected malaria patients

This study shows that HbAS and HbAC red blood cells increase Plasmodium falciparum sexual conversion rates compared to HbAA, suggesting a host genetic factor affecting malaria transmission.

Contextual control of CD8+ T cell priming by dendritic cell subsets in tumor and inflammatory microenvironments

This study shows that migratory type 1 dendritic cells are more effective than lymph node resident type 1 dendritic cells in priming CD8+ T cells in tumor and inflamed tissues.

Human lung explants as a predictive platform for evaluating the on-target, off-tumor toxicity of T cell bispecifics

This paper establishes a human lung explant model to quantify T cell bispecifics' on-target, off-tumor toxicity by measuring T cell activation and epithelial cell killing.

Fluorescence-activated cell sorting of Escherichia coli L-forms reveals maintained synchronized nucleic acid synthesis dynamics

The paper uses FACS to analyze Escherichia coli L-forms and finds they maintain synchronized nucleic acid synthesis despite lacking a cell wall.

Maleic acid adjuvates BCG to induce CD4+CD8+ T cells and sterilize tuberculosis

This study shows that maleic acid enhances BCG vaccination to induce CD4+CD8+ T cells that sterilize Mtb infection in mice.

ConvexGating infers gating strategies from clusters in single cell cytometry data

ConvexGating automatically learns unbiased, interpretable gating strategies for flow cytometry data, improving consistency and reducing contamination in cell population identification.

Targeting Tumor Immunity: Statins as a Repurposed Strategy for Preventing Ovarian Cancer Advancement

This study investigates the effect of statins on ovarian cancer survival, finding that atorvastatin improves survival and enhances immune activation in preclinical models.