Improved determination of protein turnover rate with heavy water labeling by mass isotopomer ratio selection
Jordan Currie, Dominic C. M. Ng, Boomathi Pandi, Alexander Black, Vyshnavi Manda, Cheyanne Durham, Jay Pavelka, Maggie P. Y. Lam, Edward Lau
This paper introduces a method to improve protein turnover rate calculation using heavy water labeling and mass spectrometry, increasing coverage of well-fitted peptides by 25-82% in mouse organ proteomes.
Journal of Proteome Research · Mar 18, 2025Peer-reviewedBiochemistry