Rapidly characterizing the fast dynamics of RNA genetic circuitry with cell-free transcription-translation (TX-TL) systems
Melissa K Takahashi, James Chappell, Clarmyra A Hayes, Zachary Z Sun, Vipul Singhal, Kevin J Spring, Shaima Al-Khabouri, Christopher P Fall, Vincent Noireaux, Richard M Murray, Julius B Lucks
This study uses cell-free TX-TL systems to rapidly prototype and characterize RNA genetic circuits, showing response times of ~5 minutes per cascade step and demonstrating temporal gene expression staging.
bioRxiv · Mar 18, 2014Synthetic Biology