Live-cell microscopy or fluorescence anisotropy with budded baculoviruses - which way to go with measuring ligand binding to M4 muscarinic receptors?
M.-J. Tahk, J. Torp, M. A. S. Ali, D. Fishman, L. Parts, L. Grätz, C. Müller, M. Keller, S. Veiksina, T. Laasfeld, A. Rinken
The paper compares fluorescence anisotropy and live-cell microscopy for measuring ligand binding kinetics to M4 muscarinic receptors, finding both methods suitable for affinity and kinetics analysis.
bioRxiv · Dec 22, 2021Pharmacology & Toxicology