Massively parallel quantification of substrate turnover defines protease subsite cooperativity
R. K. Gudipati, D. Gaidatzis, J. Seebacher, S. Muehlhaeusser, G. Kempf, S. Cavadini, D. Hess, C. Soneson, H. Grosshans
The paper develops qPISA to quantify protease substrate turnover, revealing subsite cooperativity in DPP4 and distinguishing it from DPF-3.
bioRxiv · Nov 17, 2023 · PreprintBiochemistry