Daniel J. Ho

DRUG-seq Provides Unbiased Biological Activity Readouts for Drug Discovery

DRUG-seq provides high-throughput, unbiased transcriptomic data for drug discovery, demonstrating reproducibility and mechanism-of-action resolution for diverse compounds.

TRAWLING: a Transcriptome Reference AWare of spLIciNG events.

TRAWLING simplifies aberrant splicing detection by providing a splicing-aware transcriptome reference, improving RNAseq analysis across multiple datasets.

Natural variation in gene expression and Zika virus susceptibility revealed by villages of neural progenitor cells

The paper identifies a common genetic variant in IFITM3 that explains most inter-individual variation in neural progenitor cell susceptibility to Zika virus using natural genetic variation and synthetic perturbations.

RNA-Seq in 296 phased trios provides a high resolution map of genomic imprinting

The paper uses RNA-Seq data from 296 family trios to identify genomic imprinting by analyzing allelic expression and parental haplotypes, finding 45 imprinted genes and one novel imprinted transcript.

Genome-wide screens in accelerated human stem cell-derived neural progenitor cells identify Zika virus host factors and drivers of proliferation

P53 toxicity is a hurdle to CRISPR/CAS9 screening and engineering in human pluripotent stem cells

This study shows that CRISPR/Cas9 induces toxicity in human pluripotent stem cells, which is dependent on TP53 and hinders genome engineering.