Efficient generation of lower induced Motor Neurons by coupling Ngn2 expression with developmental cues
Francesco Limone, Irune Guerra San Juan, Jana M. Mitchell, Janell L.M. Smith, Kavya Raghunathan, Daniel Meyer, Sulagna Dia Ghosh, Alexander Couto, Joseph R. Klim, Brian J. Joseph, John T. Gold, Curtis J. Mello, James C. Nemesh, Brittany M. Smith, Matthijs Verhage, , Kevin C. Eggan
This study combines Ngn2 overexpression with small molecule patterning to efficiently generate lower induced Motor Neurons (liMoNes) from hPSCs, achieving high yields and functional characteristics.
Cell Reports · Jan 1, 2023Peer-reviewedNeuroscience