Probing drug-target engagement of soluble Gαi1 protein using the SolThermoBRET thermal shift assay
Oscar Brod, Darya Plevako, Themiya Perera, Pattarin Hompluem, Eline J. Koers, Manuel Hilbert, David A. Sykes, Dmitry B. Veprintsev
This paper introduces a high-throughput BRET-based thermal shift assay to measure protein stability and ligand interactions with Gi1 protein without purification.
bioRxiv · Feb 15, 2024 · PreprintBiophysics