Profiling of bacterial and fungal microbial communities in cystic fibrosis sputum using RNA
N. Grahl, E. L. Dolben, L. M. Filkins, A. W. Crocker, S. D. Willger, H. G. Morrison, M. L. Sogin, A. Ashare, A. H. Gifford, N. J. Jacobs, J. D. Schwartzman, D. A. Hogan
This paper introduces a method to detect bacterial and fungal taxa in CF sputum using RNA-based NanoString probes, showing taxon-specificity and linear response over three logs of input RNA.
bioRxiv · Jun 1, 2018Microbiology