In vitro transcribed guide RNAs trigger an innate immune response via the RIG-I pathway
B. Wienert, J. Shin, E. Zelin, K. Pestal, J. E. Corn
The paper shows that in vitro transcribed guide RNAs trigger a strong innate immune response via the RIG-I pathway when used in CRISPR-Cas9 RNP editing of primary cells.
bioRxiv · Mar 3, 2018Cell Biology