Fluorescence resonance energy transfer and protein-induced fluorescence enhancement as synergetic multi-scale molecular rulers
Evelyn Ploetz, Eitan Lerner, Florence Husada, Martin Roelfes, SangYoon Chung, Johannes Hohlbein, Shimon Weiss, Thorben Cordes
This paper introduces a two-dimensional molecular ruler combining FRET and PIFE to study conformational changes in biomolecules, demonstrating its application to DNA-protein interactions and RNA polymerase dynamics.
bioRxiv · Jul 16, 2016Biophysics