SyngenicDNA: stealth-based evasion of restriction-modification barriers during bacterial genetic engineering
C. D. Johnston, S. L. Cotton, S. R. Rittling, J. R. Starr, G. Borisy, F. Dewhirst, K. P. Lemon
The paper introduces SyngenicDNA and SyMPL tools to bypass restriction-modification barriers in bacteria, achieving >100,000-fold improvement in transformation efficiency for Staphylococcus aureus.
bioRxiv · Aug 9, 2018Microbiology