Biomimetic S-Adenosylmethionine Regeneration Starting from Multiple Byproducts Enables Biocatalytic Alkylation with Radical SAM Methyltransferases
L. Gericke, L. C. Karst, D. Mhaindarkar, S. Jahn, M. Kuge, M. K. F. Mohr, J. Gagsteiger, N. V. Cornelissen, X. Wen, S. Mordhorst, H. Jessen, A. Rentmeister, F. P. Seebeck, G. Layer, C. Loenarz, J. N. Andexer
The paper develops a one-pot system to regenerate SAM and its analogues for use with methyltransferases, aminopropyl transferases, and radical SAM enzymes.
bioRxiv · Sep 26, 2022Biochemistry