X. Wen

TSAR, Thermal Shift Analysis in R, identifies endogenous molecules that interact with HIV-1 capsid hexamers

TSAR streamlines thermal shift assay analysis, identifying gallic acid and other metabolites that interact with HIV-1 capsid hexamers and may inhibit HIV-1 replication.

A Multi-task Platform for Profiling Cognitive and Motivational Constructs in Humans and Nonhuman Primates

This paper introduces a multi-task software platform for assessing cognitive and motivational functions in humans and nonhuman primates, demonstrating its use in testing NHPs on multiple tasks and extracting performance metrics.

The force-sensing GPCR LPHN2 is indispensable for normal auditory function

This study identifies LPHN2 as a force-sensing GPCR critical for auditory function, showing its role in mechanoelectrical transduction and hearing loss when deficient.

DEC1 is an adult clock regulator that links circadian rhythms to functional β-cell maturation

This study shows DEC1 regulates circadian rhythms in β-cells, linking them to metabolic maturation and glucose responsiveness.

HIV-1 resistance to islatravir/tenofovir combination therapy in wild-type or NRTI resistant strains of diverse HIV-1 subtypes

This study evaluates resistance to islatravir and tenofovir in HIV-1 strains with mutations K65R, M184V, and S68G/N, showing that their combination has a high genetic barrier to resistance.

Biomimetic S-Adenosylmethionine Regeneration Starting from Multiple Byproducts Enables Biocatalytic Alkylation with Radical SAM Methyltransferases

The paper develops a one-pot system to regenerate SAM and its analogues for use with methyltransferases, aminopropyl transferases, and radical SAM enzymes.

Non-cell-adhesive hydrogel promotes formation of human blastoid from primed human pluripotent stem cells.

A non-cell-adhesive hydrogel enables blastoid formation from primed hPSC, mimicking blastocyst structure and differentiation.

Mammary Tissue-Derived Extracellular Matrix Hydrogels Reveal the Role of Irradiation in Driving a Pro-Tumor and Immunosuppressive Microenvironment

This study shows that irradiated mammary tissue ECM hydrogels promote TNBC cell proliferation, invasion, and immunosuppression by altering ECM composition and macrophage polarization.

Mircubactin B rescues the lethal effect of cell wall biosynthesis mutations in Bacillus subtilis.

The paper identifies mirubactin B, a compound that rescues growth defects in Bacillus subtilis rod-complex mutants, showing activity beyond simple iron sequestration.

Zfp281 inhibits the pluripotent-to-totipotent state transition in mouse embryonic stem cells

This study identifies Zfp281 as a regulator that inhibits the pluripotent-to-totipotent transition in mouse embryonic stem cells by repressing transcripts upregulated during the 2C-like state transition.

MiR-144 regulates adipogenesis by mediating formation of C/EBPα-FoxO1 protein complex

This study identifies miR-144 as a regulator of adipogenesis by mediating the formation of the C/EBPα-FoxO1 protein complex, which influences AdipoQ expression and adipocyte differentiation.

Evaluation of solvents used for fabrication of microphysiological systems

This study evaluates solvent use in microphysiological systems fabrication, showing that solvent bonding harms cell cultures while photobonding avoids these issues.

In vitro nonalcoholic fatty liver disease model with cyclo-olefin-polymer-based microphysiological systems

This paper develops a COP-MPS in vitro model to study NAFLD by preventing FFA absorption, showing lipid accumulation and cell inactivation in HepaRG cells.

GPCR-mediated Activation of PLCg2 Initiates Dysregulated Recruitment of Neutrophils in Cold-induced Urticaria in PLAID Patients

This study shows that GPCR-mediated PLCγ2 activation drives neutrophil dysregulation in cold-induced urticaria in PLAID patients.

Interannual variability of net ecosystem carbon production and its climatic and biotic mechanism during 2005-2018 in a rain-fed maize ecosystem

This study examines the interannual variability of net ecosystem carbon production in a rain-fed maize ecosystem, identifying climatic and biotic factors influencing carbon dynamics from 2005 to 2018.

Cyclo olefin polymer-based solvent-free mass-productive microphysiological systems

The paper develops a cyclo olefin polymer-based microphysiological system to improve drug screening and toxicological testing by avoiding material-related issues.

Ras Inhibitor CAPRI Enables Neutrophils to Chemotax Through a Higher-Concentration Range of Gradients

This study shows that CAPRI enables human neutrophils to chemotax through a broader range of chemoattractant gradients by controlling Ras adaptation and sensitivity.

A bottom-up approach for the de novo design of functional proteins

This paper introduces a bottom-up method for designing functional proteins tailored to complex motifs, demonstrating their use as biosensors and signaling ligands.

Building a robust chromatin immunoprecipitation (ChIP) method with substantially improved efficiency

The paper develops a robust ChIP protocol that significantly improves enrichment fold in birch and Arabidopsis, and is applicable to both woody and herbaceous plants.

De novo protein design enables precise induction of functional antibodies in vivo

This paper introduces a de novo protein design algorithm called TopoBuilder that generates immunogens inducing precise antibody responses against respiratory syncytial virus in mice and non-human primates.

The LRRC8A:C Heteromeric Channel Is a cGAMP Transporter and the Dominant cGAMP Importer in Human Vasculature Cells

The paper identifies LRRC8A:C as a cGAMP transporter in human vasculature cells and shows it mediates cGAMP import/export and is the dominant importer in these cells.

Dynamics of mcr-1 prevalence and mcr-1-positive Escherichia coli after the cessation of colistin use as a feed additive for animals in China: a prospective cross-sectional and whole genome sequencing based molecular epidemiological study

This study tracks the decline of mcr-1 prevalence and genetic changes in mcr-1-positive Escherichia coli after China banned colistin use in animal feed.

TORC1 regulates vacuole membrane composition through ubiquitin- and ESCRT-dependent microautophagy

The paper shows that TORC1 inactivation leads to vacuole membrane remodeling via ubiquitination and ESCRT-dependent microautophagy.

Direct synthesis of self-organized blastocyst-like cysts derived from human pluripotent stem cells

The paper describes a method to generate self-organized blastocyst-like cysts from human pluripotent stem cells using a hydrogel matrix and identifies trophoblast and inner cell mass formation.

Single Nanoparticle Tracking Reveals Efficient Long-Distance Undercurrent Transport above Swarming Bacteria

The paper uses gold nanorods to track fluid dynamics above swarming bacteria, revealing superdiffusive transport flows that enable long-range material transport and population-level communication.

Identification, Genotyping, and Pathogenicity of Trichosporon spp. Isolated from Giant Pandas

This study identifies and genotypes Trichosporon species isolated from giant pandas, finding 11 species with varying pathogenicity and genotypes.