Goro Doi

Layer-to-cell-class correspondence is gene-specific for pyroptosis-related transcription in human skin

Epidermal NLRP1, PYCARD and CASP4 versus dermal GSDMD polarity in systemic sclerosis skin is reproduced by same-donor cell-class pseudobulk for PYCARD and GSDMD, but rarely NLRP1.

GSDMD transcript levels are higher in endothelial than cardiomyocyte compartments across three human heart cohorts

The paper compares GSDMD transcript levels between endothelial and cardiomyocyte compartments across three human heart cohorts, finding consistently higher levels in endothelial cells.

LOBSTERS: a modular vector series with a diverse set of Cas nucleases and plasmid selection markers for multiplex genome editing in Saccharomyces cerevisiae

LOBSTERS provides a modular CRISPR system with diverse Cas nucleases and plasmid markers for efficient multiplex genome editing in yeast.

Strategic targeting of Cas9 nickase expands tandem gene arrays

The paper introduces BITREx, a method using Cas9 nickase to expand tandem gene arrays in yeast and mammalian cells.

Simple-to-use CRISPR-SpCas9/SaCas9/AsCas12a vector series for genome editing in Saccharomyces cerevisiae

The paper introduces a unified set of CRISPR/Cas vectors and software for efficient genome editing in yeast using SpCas9, SaCas9, and AsCas12a.