Nanopore sequencing and assembly of a human genome with ultra-long reads
Miten Jain, Sergey Koren, Karen H. Miga, Josh Quick, Arthur C Rand, Thomas A. Sasani, John R. Tyson, Andrew David Beggs, Alexander Dilthey, Ian T. Fiddes, Sunir Malla, Hannah Marriott, Tom Nieto, Justin O’Grady, Hugh E. Olsen, , Matthew Loose
Nanopore-only sequencing of GM12878 across 39 MinION flowcells (91.2 Gb) yielded an assembly with NG50 ∼3 Mb; adding 5× ultra-long reads raised NG50 to 6.4 Mbp.
Nature Biotechnology · Jan 29, 2018Peer-reviewedGenomics✦ In-depth digest