Discovery of DTX3L inhibitors through a homogeneous FRET-based assay that monitors formation and removal of poly-ubiquitin chains
C. Vela-Rodriguez, I. Scarpulla, Y. Ashok, L. Lehtiö
This paper develops a FRET-based assay to monitor DTX3L auto-ubiquitination and identifies low micromolar inhibitors, while also expanding the system to study deubiquitinases.
bioRxiv · Mar 13, 2023Biochemistry