Restriction Endonuclease-based Modification-Dependent Enrichment (REMoDE) of DNA for Metagenomic Sequencing
S. U. Enam, J. L. Cherry, S. R. Leonard, I. N. Zheludev, D. J. Lipman, A. Z. Fire
This paper introduces a method to enrich DNA from specific organisms in metagenomic samples using restriction endonucleases and methylated motifs, achieving 11- to 142-fold enrichment for Escherichia coli and Salmonella enterica.
bioRxiv · Sep 30, 2022Microbiology