Targeted reconstruction of T cell receptor sequence from single cell RNA-sequencing links CDR3 length to T cell differentiation state
Shaked Afik, Kathleen B Yates, Kevin Bi, Samuel Darko, Jernej Godec, Ulrike Gerdemann, Leo Swadling, Daniel C Douek, Paul Klenerman, Eleanor J Barnes, Arlene H Sharpe, W. Nicholas Haining, Nir Yosef
This study develops TRAPeS, a tool to reconstruct TCR sequences from scRNA-seq data, and shows that CDR3 length correlates with T cell differentiation state in YFV-specific CD8+ T cells.
Nucleic Acids Research · Jul 17, 2017Peer-reviewedBioinformatics