Base-mismatched crRNA in CRISPR Enabled Universal One-pot Rapid Nucleic Acid Detection
Jianhao Xu, Wang Xin, Bing Yuan, Mingda Hu, Wenwen Xin, Jinglin Wang, Hongguang Ren, Yuan Yuan
This paper introduces a one-pot CRISPR-Cas12a diagnostic strategy using base-mismatched crRNA, enabling rapid and specific detection of pathogens without PAM re-design or costly modifications.
bioRxiv · Oct 1, 2026 · PreprintMolecular Biology