Author

Jitong Cai

Johns Hopkins University

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Synthetic Biology· 3 Genetics· 1 Genomics· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 4 Assay:whole-genome sequencing· 4 Research Focus:gene regulatory network inference· 3 Organism:yeast· 3 Research Focus:benchmarking· 1 Assay:bulk RNA-seq· 1 Research Focus:cell type annotation· 1 Assay:DNA methylation· 1

Frequent Co-Authors

Jef D. BoekeiD· 4 shared Joel S. BaderiD· 4 shared Yu ZhaoiD· 4 shared Giovanni StracquadanioiD· 3 shared LESLIE ANN MITCHELLiD· 3 shared Stephanie L. LaueriD· 3 shared Weimin ZhangiD· 3 shared Aaron N. BrooksiD· 2 shared

Activity by Year

2021-2023 · peak 4/yr

Co-Author Collaboration Network

10 top collaborators · 44 mutual co-author links

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4 shared papersSynthetic Biology

Jef D. BoekeORCID

Mutual co-authors in cluster

Author profile →

5 papers

Recent·Most cited

Manipulating the 3D organization of the largest synthetic yeast chromosome

Weimin Zhang, Luciana Lazar‐Stefanita, Hitoyoshi Yamashita, Michael J. Shen, LESLIE ANN MITCHELL, Hikaru Kurasawa, Evgenii Lobzaev, Viola Fanfani, Max A. B. Haase, Xiaoji Sun, Qingwen Jiang, Gregory W. Goldberg, David M. Ichikawa, Stephanie L. Lauer, Laura H. McCulloch, , Jef D. Boeke

This paper synthesizes the largest eukaryotic yeast chromosome, synIV, and investigates how its 3D structure affects gene regulation and transcription.

Molecular Cell · Nov 8, 2023Peer-reviewedSynthetic Biology

Design, Construction, and Functional Characterization of a tRNA Neochromosome in Yeast

Daniel Schindler, Roy S. K. Walker, Shuangying Jiang, Aaron N. Brooks, Yun Wang, Carolin A. Müller, Charlotte A. Cockram, Yisha Luo, Alicia García, Daniel Schraivogel, Julien Mozziconacci, Noah Peña, Mahdi Assari, María del Carmen Sánchez Olmos, Yu Zhao, , Yizhi Cai

A de novo yeast neochromosome carrying all 275 tRNA genes was built and characterized; it imposes a growth burden, with genome doubling and deletions in haploids possibly reflecting compensation.

Cell · Nov 1, 2023Peer-reviewedSynthetic Biology✦ In-depth digest

Debugging and consolidating multiple synthetic chromosomes reveals combinatorial genetic interactions

Yu Zhao, Camila Coelho, Amanda L. Hughes, Luciana Lazar‐Stefanita, Sandy Yang, Aaron N. Brooks, Roy S. K. Walker, Weimin Zhang, Stephanie L. Lauer, Cindy Hernandez, Jitong Cai, LESLIE ANN MITCHELL, Neta Agmon, Yue Shen, Joseph Sall, , Jef D. Boeke

The paper uses CRISPR D-BUGS and chromosome swapping to identify and resolve genetic interactions in synthetic chromosomes, revealing unexpected links between transcription, inositol metabolism, and tRNA abundance.

Cell · Nov 1, 2023Peer-reviewedGenomics

Synthetic chromosome fusion: effects on genome structure and function

Jingchuan Luo, Luís A. Vale-Silva, Adhithi R. Raghavan, Guillaume Mercy, Jonna Heldrich, Xiaoji Sun, Mingyu Kenneth Li, Weimin Zhang, Neta Agmon, Kun Yang, Jitong Cai, Giovanni Stracquadanio, Agnès Thierry, Yu Zhao, Camila Coelho, , Jef D. Boeke

The paper synthesizes a shorter yeast chromosome and studies its fusion with other chromosomes, revealing new 3D structural interactions and Red1 binding patterns.

bioRxiv · Aug 10, 2023 · PreprintSynthetic Biology

Priors, population sizes, and power in genome-wide hypothesis tests

Jitong Cai, Jianan Zhan, Dan E. Arking, Joel S. Bader

This paper compares the power of hypothesis testing in genome-wide studies using priors versus increasing cohort sizes, finding that larger cohorts provide greater power than strong priors.

bioRxiv · Jul 29, 2021 · PreprintGenetics