The mobility of the cap domain is essential for the substrate promiscuity of a family IV esterase from sorghum rhizosphere microbiome
M. Distaso, I. Cea-Rama, C. Coscolin, T. N. Chernikova, H. Tran, M. Ferrer, J. Sanz-Aparicio, P. N. Golyshin
This paper identifies and characterizes a novel esterase from the Sorghum bicolor rhizosphere microbiome, revealing that its cap domain mobility enables broad substrate promiscuity despite a narrow catalytic tunnel.
bioRxiv · Sep 10, 2022Biochemistry