LoxTnSeq: Random Transposon insertions combined with cre/lox recombination and counterselection to generate large random genome reductions
D. Shaw, S. Miravet-Verde, C. Pinero Lambea, L. Serrano, M. Lluch
LoxTnSeq generates genome reduction mutants by combining transposon mutagenesis, Cre/Lox recombination, and deep-sequencing, identifying 285 unique deletions in Mycoplasma pneumoniae.
bioRxiv · May 25, 2020Bioengineering