A gate and clamp regulate sequential DNA strand cleavage by CRISPR-Cas12a
M. M. Naqvi, L. Lee, O. E. Torres Montaguth, M. Szczelkun
This study investigates how CRISPR-Cas12a cleaves DNA strands sequentially by observing structural changes and using mutagenesis to show the role of a gate and clamp in the process.
bioRxiv · Jun 18, 2021 · PreprintBiochemistry