L. Lee

Prioritizing Neuroactive Ligands Using Motif-Guided Virtual Discovery and Zebrafish Profiling

The paper develops a computational method to prioritize neuroactive ligands using structural data and zebrafish testing, identifying effective antagonists for hypocretin receptors.

Rapid in situ mutation detection in extracellular vesicle-DNA

This paper introduces a rapid, PCR- and sequencing-free DNA mutation detection method using CRISPR-Cas12a and extracellular vesicles for cancer diagnosis and treatment monitoring.

Epigenetic coordination of transcriptional and translational programs in hypoxia

The paper shows hypoxia induces TSS switching via H3K4me3 changes, altering translation of key metabolic genes like PDK1.

Do working forest easements work for conservation?

This study evaluates the effectiveness of working forest conservation easements in Maine, finding minimal ecological benefits and increased harvesting rates.

Structural Optimization of siRNA Conjugates for Albumin Binding Achieves Effective MCL1-Targeted Cancer Therapy

This paper optimizes siRNA conjugates for albumin binding to enhance tumor delivery and efficacy in cancer therapy, achieving 80% MCL1 silencing and improved survival in breast cancer models.

Network analysis with either Illumina or MinION reveals that detecting vertebrate species requires metabarcoding of iDNA from a diverse fly community

The paper uses metabarcoding of iDNA from dung and carrion flies to detect vertebrate species in a swamp forest, finding that 18 of 20 vertebrate species are detectable within 150m of a road.

Stem cell regulators control a G1 duration gradient in the plant root meristem

The paper shows that stem cell regulators create a G1 duration gradient in the plant root meristem, with mutants disrupting this gradient.

Mechanisms underlying sequence-dependent DNA hybridisation rates in the absence of secondary structure

The paper measures DNA hybridisation rates of sequences without secondary structure and develops a model based on complementary nucleating interactions and sequence-dependent stability.

Riding into the future on a fly: toward a better understanding of phoresy and avian lice evolution (Phthiraptera) by screening bird carcasses for phoretic lice on hippoboscid flies (Diptera)

The paper uses NGS barcoding to study phoretic relationships between avian lice and hippoboscid flies by screening bird carcasses, discovering new phoresy cases and interaction networks.

A gate and clamp regulate sequential DNA strand cleavage by CRISPR-Cas12a

This study investigates how CRISPR-Cas12a cleaves DNA strands sequentially by observing structural changes and using mutagenesis to show the role of a gate and clamp in the process.

Influence of substrate stiffness on barrier function in an iPSC-derived in vitro blood-brain barrier model

This study examines how substrate stiffness affects barrier function in iPSC-derived BBB endothelial-like cells, finding enhanced TEER on compliant substrates and no effect on P-glycoprotein activity.

MinION barcodes: biodiversity discovery and identification by everyone, for everyone

This paper introduces a low-cost, accessible workflow and software for DNA barcoding using MinION sequencing, achieving high accuracy comparable to Sanger and Illumina methods.

Doublecortin engages the microtubule lattice through a cooperative binding mode involving its C- terminal domain

This study reveals how doublecortin binds to microtubules through a cooperative mechanism involving its C-terminal domain and C-tails, with implications for neurological disorders.

BMP pathway antagonism by Grem1 regulates epithelial cell fate in intestinal regeneration

This study shows that Grem1 antagonizes BMP signaling to regulate epithelial cell fate during intestinal regeneration.

An in vivo CRISPR screening platform for prioritizing therapeutic targets in AML

This study identifies novel therapeutic targets for AML using an in vivo CRISPR screening platform, revealing SLC5A3 as a metabolic vulnerability and MARCH5 as an apoptosis regulator.

Evaluations of CRC2631 toxicity, tumor colonization, and genetic stability in the TRAMP prostate cancer model

This study evaluates the toxicity, tumor colonization, and genetic stability of Salmonella enterica serovar Typhimurium CRC2631 in a TRAMP prostate cancer model and shows its potential for cancer immunotherapy.

Bromodomain and Extraterminal Inhibition Blocks Inflammation-Induced Cardiac Dysfunction and SARS-CoV-2 Infection (Pre-Clinical)

The paper identifies BET inhibitors as potential cardio-protective drugs that block inflammation-induced cardiac dysfunction and SARS-CoV-2 infection in pre-clinical models.

Spatial simulation of co-designed land-cover change scenarios in New England: Alternative futures and their consequences for conservation priorities

The paper translates participatory land-use scenarios into spatial simulations to assess future landscape conditions and their impact on conservation priorities in New England.

Parkin-mediated ubiquitination contributes to the constitutive turnover of mitochondrial fission factor (Mff)

This study shows Parkin ubiquitinates Mff under basal conditions to regulate its constitutive turnover, independent of PINK1 and stress.

Translational control of breast cancer plasticity

The paper identifies hypoxia and drug-induced translational activation of NANOG, SNAIL, and NODAL isoforms that drive breast cancer plasticity and stem-cell-like phenotypes, with potential therapeutic implications.

Protective role of the deSUMOylating enzyme SENP3 in myocardial ischaemia-reperfusion injury

This study shows that SENP3 levels decrease during cardiac ischaemia and reperfusion, and its loss increases cell death, suggesting a protective role in myocardial injury.

Variation in traction forces during cell cycle progression

The paper measures traction forces during the cell cycle and finds they increase in G1, plateau in S phase, and decrease in G2, revealing a previously unknown regulation of cell contractility.