Systematic De-Risking of TCR-Mimic Therapeutics Through Proteome-Wide Off-Target Landscaping and a Generalizable Design Rule Framework
Simon Schuster, Frederike A. Hartl, Jennifer Stehle, Fabian Beier, Hoor Al-Hasani, Christoph Reinhart, Tsai‐Hsuan Weng, Stefan Krämer, Pranav Hamde, Tobias Herz, Sebastian Oesterlin, Marco Schmidt, Tabea Gross, Luca Stehl, Normann Kilb, , Jörg Birkenfeld
This paper introduces ValidaTe, a framework to predict and mitigate off-target liabilities in TCR-mimic antibodies, demonstrating its effectiveness with CTA MAGE-A4 targets.
bioRxiv · Aug 4, 2026 · PreprintImmunology