Engineering a super-secreting strain of Escherichia coli by directed co-evolution of the multiprotein Tat translocation machinery
May N. Taw, Mingji Li, Daniel Kim, Mark A. Rocco, Dujduan Waraho‐Zhmayev, Matthew P. DeLisa
The paper evolves Escherichia coli Tat translocases to enhance protein secretion, finding three variants that significantly increase periplasmic protein export compared to wildtype and SecYEG.
ACS Synthetic Biology · Nov 10, 2021Peer-reviewedSynthetic Biology