From hours to days: prolonging the functional viability of acute brain slices to >36 h
Elizaveta N. Vylekzhanina, Luca Habelt, Ashlyn Creamer, Christian Cameron de Abos y Padilla, Ilka Diester, Michael Niessing, Peter Hochstrate, Philippe Coulon
This paper introduces an experimental protocol that extends the viability of acute brain slices beyond 36 hours, enabling long-term electrophysiological studies with reduced animal use and cost.
bioRxiv · Oct 24, 2026 · PreprintNeuroscience