Characterization of transcript enrichment and detection bias in single-nuclei RNA-seq for mapping of distinct human adipocyte lineages
A. Gupta, F. Shamsi, N. Altemose, G. F. Dorlhiac, A. M. Cypess, A. P. White, M. E. Patti, Y.-H. Tseng, A. M. Streets
This study compares snRNA-seq and scRNA-seq in adipose tissue, showing snRNA-seq can identify cell types but has transcript enrichment and detection biases that require normalization.
bioRxiv · Mar 24, 2021Bioengineering