Efficient and rapid fluorescent protein knock-in with universal donors in mammalian stem cells
Y. Shi, N. Kopparapu, L. Ohler, D. J. Dickinson
This paper describes a method for efficiently inserting fluorescent proteins into endogenous genes in mammalian stem cells using NHEJ, reducing the time from transfection to imaging to less than two weeks.
bioRxiv · Oct 10, 2022 · PreprintCell Biology