Light induced expression of gRNA allows for optogenetic gene editing of T lymphocytes in vivo
D. A. Velasquez Pulgarin, N. Pelo, L. Ferrandiz, T. Trselic, W. A. Nyberg, G. L. Bowlin, A. Espinosa
This paper introduces BLU-VIPR, an optogenetic CRISPR tool that enables precise spatiotemporal gene editing in T lymphocytes in vivo by regulating gRNA production with blue light.
bioRxiv · Nov 9, 2023Bioengineering