Targeted mutagenesis of the Arabidopsis GROWTH-REGULATING FACTOR (GRF) gene family suggests competition of multiplexed sgRNAs for Cas9 apoprotein
J. Angulo, C. P. Astin, O. Bauer, K. J. Blash, N. M. Bowen, N. J. Chukwudinma, A. S. Dinofrio, D. O. Faletti, A. M. Ghulam, C. M. Gusinde-Duffy, K. J. Horace, A. M. Ingram, K. E. Isaack, G. Jeong, R. I. Kiser, , W. Lukowitz
The paper develops CRISPR/Cas9 vectors for plant genome editing and finds that multiplexing sgRNAs reduces mutation rates at some loci due to Cas9 competition.
bioRxiv · Aug 16, 2020 · PreprintPlant Biology