Plant BiologybioRxivPreprint
Targeted mutagenesis of the Arabidopsis GROWTH-REGULATING FACTOR (GRF) gene family suggests competition of multiplexed sgRNAs for Cas9 apoprotein
J. Angulo, C. P. Astin, O. Bauer…·W. Lukowitz
The paper develops CRISPR/Cas9 vectors for plant genome editing and finds that multiplexing sgRNAs reduces mutation rates at some loci due to Cas9 competition.
DOI 10.1101/2020.08.16.253203