Enzyme Intermediates Captured "on-the-fly" by Mix-and-Inject Serial Crystallography
J. Olmos, S. Pandey, J. M. Martin-Garcia, G. Calvey, A. Katz, J. Knoska, C. Kupitz, M. S. Hunter, M. Liang, D. Oberthuer, O. Yefanov, M. Wiedorn, M. Heymann, M. Holl, K. Pande, , Our
This paper demonstrates a method to capture enzyme reactions in real-time using mix-and-inject serial crystallography, revealing antibiotic cleavage and kinetic transitions in Mycobacterium tuberculosis β-lactamase.
bioRxiv · Jan 18, 2018Biophysics