Measuring conformational equilibria in allosteric proteins with time-resolved tmFRET
W. N. Zagotta, E. G. Evans, P. Eggan, M. Tessmer, K. D. Shaffer, J. Petersson, S. Stoll, S. E. Gordon
This paper develops a fluorescence method to measure protein conformational dynamics and energetics using time-resolved tmFRET and validates it with EPR spectroscopy.
bioRxiv · Oct 9, 2023Biophysics