Optimizing mouse metatranscriptome profiling by selective removal of redundant nucleic acid sequences
Morgan Roos, Samuel Bunga, Asako Tan, Erica S. Maissy, Dylan Skola, Alexander Richter, Daniel S. Whittaker, Paula Desplats, Amir Zarrinpar, Rick Conrad, Scott Kuersten
The paper improves rRNA depletion for mouse cecal metatranscriptome analysis by designing probes based on sequence abundance rather than taxonomy, increasing mRNA reads and reducing bias.
mSystems · Jun 16, 2025Peer-reviewedGenomics