Author

Ruhani Makkar

John Radcliffe Hospital

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Bioengineering· 2 Synthetic Biology· 1

Signature Assays, Organisms & Methods

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Research Focus:benchmarking· 1 Assay:bulk RNA-seq· 1 Research Focus:cell type annotation· 1 Approach:experimental assay· 1 Approach:machine learning (classical)· 1 Organism:mouse· 1 Research Focus:protein design· 1 Approach:statistical model· 1

Frequent Co-Authors

Dhanu GuptaiD· 3 shared Harrison SteeliD· 2 shared Ioulia VorobievaiD· 2 shared Marina LuchneriD· 2 shared Simon ChesteriD· 2 shared Stephan J. SandersiD· 2 shared André GörgensiD· 1 shared Antje Maria ZickleriD· 1 shared

Activity by Year

2025-2026 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 25 mutual co-author links

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3 shared papersBioengineering

Dhanu GuptaORCID

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Author profile →

3 papers

Recent·Most cited

Machine learning-assisted directed evolution yields dramatic improvement on novel AAV engineering task

Marina Luchner, Ioulia Vorobieva, Ruhani Makkar, Mattea Jozinovic, Simon Chester, Matthew J A Wood, Stephan Sanders, Dhanu Gupta, Harrison Steel

This study shows ML-assisted directed evolution improves AAV capsid manufacturing efficiency 41-fold compared to directed evolution alone.

bioRxiv · Oct 7, 2026 · PreprintBioengineering

Systemic Inflammation Modulates Clearance and drives Extra-Hepatic Distribution of Extracellular Vesicles

Svetlana Pavlova, Doste Rashid Mamand, André Görgens, Antje Maria Zickler, Wenyi Zheng, Xiuming Liang, Oscar P. B. Wiklander, Manuchehr Abedi‐Valugerdi, Elien Van Wonterghem, Junhua Xie, Zheyu Niu, Samantha Roudi, Dongnan Yan, Ruhani Makkar, Guannan Zhou, , Samir EL Andaloussi

This study shows that systemic inflammation significantly prolongs EV plasma half-life and enhances their accumulation in inflamed organs like the liver and spleen.

Journal of Extracellular Vesicles · Apr 1, 2026Peer-reviewedBioengineering

Engineered AAV Capsids with Enhanced Extracellular Vesicle Loading via Rational Design and Directed Evolution

Ioulia Vorobieva, Marina Luchner, Hans Friedrichsen, Ruhani Makkar, Simon Chester, Emily Haughton, Mariana Conceiao, Harrison Steel, Stephan J. Sanders, Matthew PA Wood, Dhanu Gupta

The paper engineers AAV9 capsids to enhance extracellular vesicle loading through rational design and directed evolution, achieving up to threefold increased EV-AAV yield and improved transduction efficiency.

bioRxiv · Dec 13, 2025 · PreprintSynthetic Biology