Quantitative fluorescence lifetime imaging of protein microenvironments by genetically encoded compact benzodiazole probes
Haocheng Qianzhu, Stephen M. Butler, Edan Habel, Elwy H. Abdelkader, Nathan Paul, Ryan Woolsey, Xuanhui Zhou, Rory M. Martin, Katrina A. Jolliffe, Gottfried Otting, Thomas Huber
This paper introduces genetically encoded benzodiazole-based probes for quantitative FLIM, enabling site-specific analysis of protein microenvironments in living cells.
bioRxiv · Oct 17, 2026 · PreprintBiophysics