Genome-wide Characterization of Diverse Bacteriophages Enabled by RNA-Binding CRISPRi
B. A. Adler, M. J. Al-Shimary, J. R. Patel, E. Ambruster, D. Colognori, E. J. Charles, K. V. Miller, A. Lahiri, M. Trinidad, R. Boger, J. Nomburg, S. Beurnier, M. L. Cui, R. Barrangou, V. K. Mutalik, , B. F. Cress
The paper develops a CRISPRi-ART system to knock down protein expression in diverse bacteriophages and identifies essential genes, including many with unknown functions.
bioRxiv · Sep 18, 2023 · PreprintMicrobiology